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Spectrophotometers
UV-VIS-IR

Advanced Analysis of Scattering Media

SphereSpectro 150H Spectrophotometer: Advanced Analysis of Scattering Media

Patented Spectrophotometer for determining the spectral absorption coefficient and effective scattering coefficient of scattering or turbid media.

  • Spectrophotometer for determining the spectral absorption coefficient and effective scattering coefficient of scattering media
  • For clear samples the classical 8°/d measurement geometry is supported
  • Different stages of expansion in the spectral range UV, VIS to IR
  • Patented Measuring System

The SphereSpectro 150H stands out as an innovative spectrophotometer system designed for the concurrent measurement of both the spectral absorption coefficient and the spectral effective scattering coefficient in scattering media (patented). The device is available in various models suitable for UV, VIS, and IR spectral ranges, and it can employ the classical 8°/d measurement geometry for clear samples.

ModelSpectral range
SphereSpectro 150H-V01 350 nm – 1050 nm
SphereSpectro 150H-V02 350 nm – 2150 nm
SphereSpectro 150H-V03200 nm – 2150 nm

Key Features of the SphereSpectro 150H

Simultaneous Measurement of 2 coefficients

Absorption coefficient, µa
effective scattering coefficient, µs

Versatile Sample Measurement:

Measurement on diffuse sample, both solid and liquid
Measurement in classical 8°/d measurement geometry for clear probes (according to DIN 5036-3 as well as CIE-130-1998)

User-Friendly Operation:

Simple sample handling
Rapid measurements within seconds
Compact, table-top design
Compatibility with UV, VIS, and IR spectral ranges
Spacious sample compartment with multiple fixing options
High precision and absolute measurement capability
Intuitive plug-and-play software package

Measurement Principle and Applications

The SphereSpectro 150H employs a fundamental principle allowing the determination of both the spectral absorption coefficient and the spectral effective scattering coefficient, essential for analyzing diffuse scattering samples’ physical and chemical properties. Unlike traditional spectrophotometers that focus solely on absorption or transmission, the SphereSpectro 150H provides comprehensive analysis, making it indispensable for applications requiring absolute measurements.

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Applications

Material Analysis
Biophotonics
Active Ingredient Determination
Quality Assurance
Chemometrics
Food Analysis
Pharmaceuticals and Cosmetics
Physical Parameter-Based Rendering

For instance, the device can be used to evaluate the aging behavior of metalworking fluids by simultaneously determining droplet size, pH value, and concentration. It also aids in process control by measuring effective scattering coefficients to determine the fat content in homogenized milk through multivariate data modeling.

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Advantages Over Typical Spectrophotometers

Conventional spectrophotometers struggle with scattering media as they primarily measure transmitted light and fail to distinguish between scattering and absorption. The SphereSpectro 150H, however, excels in this area by measuring absolute values, thus enabling a detailed analysis of physical and chemical material properties. This capability is crucial for applications like determining concentration levels or evaluating material properties in cross-linking processes.

Ease of Sample Preparation

The SphereSpectro 150H simplifies sample preparation compared to traditional spectrophotometers. Solid translucent samples are easily held using the sample holder, while liquid samples can be conveniently filled into cuvettes provided with the system, eliminating the need for special pre-treatment.

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Measuring Scattering Media

Scattering media contain areas with varying refractive indices, causing light to scatter in multiple directions. This scattering results in diffuse reflectance and total reflection, which includes directional reflection at the medium’s boundary. Additionally, total transmission comprises collimated transmission (direct light passage) and diffuse transmission (light that has interacted with the medium).

Optical Property Determination with an Integrating Sphere

An integrating sphere is used to measure the total reflection and transmission of scattering samples, integrating radiation over the entire sample surface. By comparing these measurements with theoretical models, the spectral absorption and scattering coefficients can be determined. The software provided with the SphereSpectro 150H includes a robust algorithm for precise simulation of light propagation within the integrating sphere. Users can input parameters such as sample thickness and refractive index, and the automated evaluation process takes seconds to minutes.

For more information on the measurement techniques and applications, refer to our scientific publications on the theory and experiments related to the absorption and scattering of diffuse media. Contact us for further details or specific measurement needs.

Check how it works

SphereSpectro – UV-VIS-IR Spectrophotometer

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