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Nikon Microscope Objectives

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Exploring Microscope Objectives

Optics play a crucial role in both cameras and microscopes, serving as the primary components that focus and magnify light to create clear and detailed images. Despite their shared fundamental principles, the objectives and lenses in these devices are tailored to meet distinct requirements based on their specific applications. This article delves into the objectives and lenses used in cameras and microscopes, highlighting their objectives, design, and functionalities.

Microscope objectives aim to magnify tiny specimens while maintaining high resolution and clarity. These lenses are crucial in scientific research and medical diagnostics, where observing fine details is essential.

Types of

Microscope Objectives

  1. CFI60 LE Plan EPI Brightfield Infinity Corrected Objectives
  2. CFI60-2 TU Plan EPI Fluor Brightfield/Darkfield Infinity Corrected Objectives
  3. Achromatic Finite Conjugate Objectives
  4. CFI Plan Fluor Objectives
  5. CFI Super Fluor Objectives
  6. CFI60 Infinity Corrected Brightfield Objectives

Nikon CFI60 LE Plan EPI Brightfield Infinity Corrected Objectives

Nikon CFI60 LE Plan EPI Brightfield Infinity Corrected Objectives are high-performance microscope objectives engineered for industrial brightfield microscopy within Nikon’s CFI60 optical system, which combines infinity optics with the superior image quality of the CF system. They offer long working distances together with high numerical apertures (NA), ensuring clear, high-contrast, and high-resolution imaging across a flat field.


  • High NA, enabling superior resolution and fine-detail imaging in brightfield microscopy.
    Long Working Distances, providing sample clearance suitable for a variety of industrial inspection tasks.
  • Parfocal Distance of 60 mm, ensuring consistent focus when switching between magnifications and maintaining image clarity.
  • Plan Optical Design, delivering sharp, flat images across the entire field of view.
  • Magnification Range, available from 5X to 50X to suit diverse observation and measurement needs.
  • Applications: technical cleanliness evaluation, steel quality rating, metallography, and other industrial material inspections requiring precision brightfield imaging.

CFI60-2 TU Plan EPI Fluor Brightfield/Darkfield Infinity Corrected Objectives

Nikon CFI60-2 TU Plan EPI Fluor Brightfield/Darkfield Infinity Corrected Objectives are precision-engineered microscope objectives designed for dual brightfield and darkfield imaging in applications that demand a long working distance and high optical performance.

  • Nikon Unique Fresnel Lens, enabling highly effective chromatic aberration correction for sharper, clearer images across the visible spectrum.
  • Highly Corrected Chromatic Aberration, ensuring accurate color reproduction and improved resolution in both brightfield and darkfield modes.
  • Long Working Distance, providing ample clearance between lens and specimen for large or delicate samples in industrial and biological imaging.
  • Plan Optical Design, delivering flat-field images with excellent contrast and resolution across the full field of view.
  • Magnification Range, available from 5X to 100X to suit a wide range of inspection and analytical needs.
  • Applications: industrial metrology, brightfield and darkfield inspection, biological imaging requiring precise chromatic correction, and high-contrast observation in both incident and scattered light modes.

Achromatic Finite Conjugate Objectives

Nikon Achromatic Finite Conjugate Objectives are cost-effective, high-performance microscope objectives designed for simple high-power imaging systems without the need for a tube lens. They feature a classic achromatic optical design that corrects for chromatic aberrations in red and blue wavelengths, ensuring clear, sharp images for routine microscopy applications.

  • Achromatic Design, providing good color correction and image clarity at an excellent performance-to-cost ratio.
  • Finite Conjugate Optics, enabling direct interfacing with an eyepiece or camera without a tube lens; compatible with DIN standard microscopes.
  • Standard RMS Thread, allowing quick, easy integration with existing systems and broad accessory compatibility.
  • Spring Loaded Heads on 40X and 60X objectives, protecting the lens and specimen from accidental contact damage.
  • Magnification Options, available in 4X, 10X, 40X, and 60X for a range of imaging needs.
  • Applications: general laboratory work, educational microscopy, simple imaging setups, and any application requiring reliable optical performance at low cost with easy mechanical integration.

CFI Plan Fluor Objectives

Nikon CFI Plan Fluor Objectives are versatile, high-performance microscope objectives designed to provide high contrast fluorescence observation with exceptional optical quality. These objectives feature broad transmission from the ultraviolet (UV) to infrared (IR) regions, making them suitable for a wide range of advanced microscopy techniques.

  • High Transmission from UV to IR, enabling efficient light throughput for fluorescence and other contrast-enhancing imaging methods.
  • Exceptional Flatness Across the Field of View, delivering sharp, distortion-free images suitable for quantitative and high-resolution imaging.
  • Multipurpose Use, ideal for brightfield, fluorescence, polarizing, and differential interference contrast (DIC) microscopy.
  • High Contrast Fluorescence Observation, supporting sensitive detection and accurate imaging of fluorescent samples.
  • Mounting with M25 x 0.75 threads for easy integration into compatible microscopy systems.
  • Magnification Range from 4X to 60X, covering low to medium magnification needs in various microscopy applications.
  • Applications: fluorescence microscopy, photomicrography, polarized light studies, DIC microscopy, and general brightfield imaging in research, clinical, and industrial settings.

CFI Super Fluor Objectives

Nikon CFI Super Fluor Objectives are specialized microscope objectives designed for superior ultraviolet (UV) transmission and optimized fluorescence imaging at short wavelengths down to 340 nm. They are ideal for demanding applications involving UV-excited fluorochromes and photo-activation processes.

  • Superior UV Transmission, enabling efficient imaging and excitation at wavelengths as short as 340 nm, suitable for fluorochromes such as indo-1, fura-2, and fluo-3.
  • High Numerical Aperture Designs, providing bright, sharp fluorescence images with enhanced resolution and light-gathering capability.
  • Low Auto-Fluorescence Materials, reducing background noise and improving signal-to-noise ratio for clearer and more accurate fluorescence imaging.
  • Optimized for Improved Signal-to-Noise Ratios at short UV wavelengths, ensuring precise detection of fluorescence signals.
  • Applications: UV fluorescence microscopy, live-cell imaging with UV-excited fluorochromes, photo-activation of caged compounds, and other advanced fluorescence techniques requiring high sensitivity and resolution in the UV spectrum.

CFI60 Infinity Corrected Brightfield Objectives

Nikon CFI60 Infinity Corrected Brightfield Objectives are advanced microscope objectives developed to meet demanding imaging requirements, representing the culmination of Nikon’s optical engineering. They combine long working distance with high numerical aperture (NA) for maximum light-gathering ability, thanks to their 60 mm parfocal distance and large barrel diameter.

  • Excellent Color Reproduction, delivering accurate, true-to-life images for precise visual analysis.
  • Long Working Distance and High NA, optimizing both sample clearance and image brightness/resolution.
  • High Contrast with Minimal Flare, enabling sharp and well-defined images across the entire field of view.
  • Strain Free optical design suitable for applications where optical stress could otherwise distort images.
  • Advanced Aberration Correction, compensates for lateral and axial chromatic aberrations over the full field for high clarity and resolution.
  • Magnification Range: available from 5X to 100X for a broad spectrum of brightfield imaging needs.
  • Applications: high-precision brightfield microscopy in research, clinical, and industrial settings; material and biological imaging requiring accurate color rendering, high resolution, and minimized optical strain.

SEARCH:

  • Choose a specific value to narrow down the table.
  • The match must be exact
  • For example: “UV”, “HR”, “100X”, “APO”
ItemModelStyleMagnificationNumerical Aperture NA:Field Number (mm):Focal Length (mm)Working Distance (mm):Specification
Nikon
MTI LE Plan BF MUD00055
MTI LE Plan BF MUD00055
#29-220
Infinity Corrected50.12231.0Specification
Nikon
MTI LE Plan BF MUD00105
MTI LE Plan BF MUD00105
#29-221
Infinity Corrected100.252213.0Specification
Nikon
MTI LE Plan BF MUD00055
MTI LE Plan BF MUD00205
#29-222
Infinity Corrected200.4223.6Specification
Nikon
MTI LE Plan BF MUD00505
MTI LE Plan BF MUD00505
#29-223
Infinity Corrected500.75220.5Specification
Nikon
TU Plan EPI Fluor BD MUE42050
TU Plan EPI Fluor BD
MUE42050
#29-224
Infinity Corrected5X0.1540.0018.0Specification
Nikon
TU Plan EPI Fluor BD MUE42100
TU Plan EPI Fluor BD
MUE42100
#29-225
Infinity Corrected10X0.3020.0015.0Specification
Nikon
TU Plan EPI Fluor BD MUE42200
TU Plan EPI Fluor BD
MUE42200
#29-226
Infinity Corrected20X0.4510.004.5Specification
Nikon
TU Plan EPI Fluor BD MUE42500
TU Plan EPI Fluor BD
MUE42500
#29-227
Infinity Corrected50X0.804.01.0Specification
Nikon
TU Plan EPI Fluor BD
MUE42900
#29-228
Infinity Corrected100X0.902.01.0Specification
Nikon
Achromatic MSB50040
Achromatic
MSB50040
#59-934
Finite Conjugate4X0.101825.0Specification
Nikon
Achromatic MSB50100
Achromatic
MSB50100
#59-935
Finite Conjugate10X0.25185.6Specification
Nikon
CFI Plan Fluor MRH00045
CFI Plan Fluor
MRH00045
#88-378
Infinity Corrected4X0.132517.2Specification
Nikon
CFI Plan Fluor MRH00105
CFI Plan Fluor
MRH00105
#88-379
Infinity Corrected10X0.302516.0Specification
Nikon
CFI Plan Fluor MRH00205
CFI Plan Fluor
MRH00205
#88-380
Infinity Corrected20X0.50252.1Specification
Nikon
CFI Plan Fluor
CFI Plan Fluor
MRH00401
#88-381
Infinity Corrected40X0.7525.20.66Specification
Nikon
CFI Plan Fluor MRH00602
CFI Plan Fluor
MRH00602
#88-382
Infinity Corrected60X0.8525.20.3Specification
Nikon
Nikon CFI Super Fluor MRF00101
CFI Super Fluor
MRF00101
#88-375
Infinity Corrected10X0.50221.2Specification
Nikon

Nikon CFI Super Fluor MRF00201
CFI Super Fluor
MRF00201
#88-376
Infinity Corrected20X0.75221.0Specification
Nikon
CFI60 Infinity Corrected Brightfield Nikon MUE12050
CFI60 Brightfield
MUE12050
#58-515
Infinity Corrected5X0.152240.0023.5Specification
Nikon
CFI60 Infinity Corrected Brightfield Nikon MUE12100
CFI60 Brightfield
MUE12100
#58-516
Infinity Corrected10X0.302220.0017.5Specification
Nikon
CFI60 Infinity Corrected Brightfield Nikon MUE12200
CFI60 Brightfield
MUE21200
#58-517
Infinity Corrected20X0.402210.0019.00Specification
Nikon
CFI60 Infinity Corrected Brightfield Nikon MUE12500
CFI60 Brightfield
MUE21500
#58-518
Infinity Corrected50X0.60224.0011.00Specification
Nikon
CFI60 Infinity Corrected Brightfield Nikon MUE21900
CFI60 Brightfield
MUE21900
#58-519
Infinity Corrected100X0.80222.004.5Specification

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