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Luty 2020

ANSYS LUMERICAL
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LUMERICAL jest obecnie częścią ANSYS. SPECTROPOL pozostał nadal jedynym dystrybutorem produktów Lumericala w Polsce. Zapraszamy

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Działamy na rynku od 18 lat i stale się rozwijamy.

Naszym celem jest współpraca z najlepszymi w branży dostawcami, dzięki którym zapewniamy Państwu dostęp do najlepszych i sprawdzonych produktów oraz technologii. 

WYDARZENIA 2021

  • ANSYS Simulation World +

    ANSYS Simulation World Zapraszamy 20-21.04.2021 na największe wydarzenie tego rodzaju, które ma inspirować i kształcić kadrę kierowniczą, inżynierów, specjalistów ds. badań i rozwoju Read More
  • SPIE Photonics West - Maj +

    SPIE Photonics West - Maj On-line, 9-11-Maj 2021, San Francisco, USA
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    AUTOMATICON  - Maj 19-21 Maj 2021 Warszawa Read More
  • Laser World of Photonics - Czerwiec +

    Laser World of Photonics - Czerwiec 21.06-24.06.2021 Read More
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    EUROLAB - Brak informacji od organizatora ! Warszawa, Targi i Konferencja Read More
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NAJNOWSZE WIADOMOŚCI

  • Nowe partnerstwo

    Nowe partnerstwo

    W 2020 Lumerical dołaczył do grupy ANSYS. Zmianie uległy jedynie plany taryfowe dla korporacji nieakademickich. Read More
  • KAMERA pco.dicam C1

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    Kamera ze wzmocnieniem obrazu 25mm, sCMOS, 16 bit, czas ekspozycji 4 ns - 1 s Read More
  • KORONAWIRUS

    KORONAWIRUS

    SPECTROPOL został ekskluzywnym dystrybutorem testów do wykrywania koronowirusa amerykańskiej firmy Co-Diagnostics. Read More
  • MODULATOR PEM 200

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    Nowy cyfrowy modulator fotoelastyczny pozwala na modulację ( przy stałej częstotliwości) polaryzacji wiązki światła w zakresie UV-VIS-IR. Read More
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  • W 2020 r Lumerical dołaczył do grupy Ansys. Zmianie uległy jedynie plany taryfowe dla klientów komercyjnych.   
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  •                 Purchase includes: Pocket NC V2-50 5 Axis Desktop CNC Mill CHB 1/8” Collet 1/8th Inch Square Plastic Cutting Endmill, Single Flute One year subscription of Autodesk Fusion 360 Ultimate™ Regulator for air, fittings, air cleaning kit Pocket NC vise and hardware USB cable, Power cord, Pocket NC Limited Warranty     V2-50 Specification Download
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  • Jednokanałowy system wysokiej rozdzielczości (0,15nm) dostosowany zarówno do analizy spektralnej plazmy jak i monitoringu procesu.  
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  • W ofercie SPECTROPOLU zestawy Logix Smart Coronavirus disease 2019 (COVID-19),  testy diagnostyczne in vitro oparte na technologii PCR w czasie rzeczywistym (qPCR), służące do jakościowego wykrywania RNA z SARS-CoV-2 (COVID-19), metodą zalecaną przez WHO (WHO, 2020) i US CDC (CDC, 2020).
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  • kompaktowe wymiary: 65 x 65 x 65 mm³ back illuminated 16 bit sCMOS sensor zakres 190-1100 nm wysoka rozdzielczość 2048 x 2048 pikseli wymiar piksela: 6.5 x 6.5 µm² wydajność kwantowa do 95 % czas naświetlania 100 µs do 1 s maksymalna liczba klatek na sekundę 40 fps @ pełna rozdzielczość rolling shutter interfejs USB 3.1 Gen1 zasilanie przez USB  
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Ciekawe zastosowania

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Optical O2 and pH Sensors for Bioreactor Environments

Advances in sensor materials and optoelectronics have enabled novel optical sensors for applications in life sciences, pharmaceuticals, biotechnology and more. Compared with electrochemical sensing techniques such as galvanic sensors, optical sensors can be made in small and customizable form factors, allow non-intrusive measurements and do not consume the sample.

The principle of operation is to trap an oxygen-sensitive fluorophore or pH indicator dye in a host matrix (polymer for oxygen and sol-gel for pH) that is applied to the tip of a fiber, to an adhesive membrane such as a patch, or to a flat substrate such as a microtiter plate. The indicator materials change optical properties in response to specific analytes and electronics measure the response. For oxygen, a phase fluorometer measures the partial pressure of dissolved or gaseous oxygen; for pH, a miniature spectrometer measures the colorimetric (absorbance) response of the pH dye.

To demonstrate the viability of optical oxygen and pH sensors for monitoring biological parameters in bioreactor environments, we placed oxygen- and pH-sensitive adhesive patches inside a bioflask to monitor conditions during an E. coli fermentation reaction. Bioreactors are closed-environment systems where the cells are cultured under specific conditions to synthesize the final product. Such systems require constant monitoring of DO and pH to optimize the bioprocesses. Optical sensors were used to provide oxygen and pH measurements in the liquid phase of the bioreactor.

Monitoring of E. coli Fermentation Processes

Fermentation of E. coli for the production of recombinant proteins and other products is a complex, multistep process that requires rigorous control of culture conditions. For optimal fermentation to take place in the bioreactor, oxygen and pH levels must be maintained within a narrow range. The bacterial cells consume oxygen as they replicate, so the culture must be sparged with oxygen as necessary. Also, the bacterial cells release metabolic by-products during the growth process, requiring periodic rebalancing of pH levels. Maintaining optimal conditions to ensure high yields requires frequent monitoring of oxygen and pH in real time during the reaction.

Various technologies exist for oxygen and pH measurements, including galvanic sensors, paramagnetic sensors, fuel-cell sensors and even paper. Yet all these techniques are invasive and require sampling the culture or placing a probe or sensor into the reactor vessel, which can introduce contamination or alter growth conditions.

Optical sensors provide noncontact alternatives to more invasive monitoring methods. Fluorescence can be used to monitor oxygen in the liquid and headspace phases of the bioreactor. Partial pressure of dissolved or gaseous oxygen is monitored using a phase fluorometer to measure the phase shift between the excitation signal of a blue LED and the emission signal of the fluorescence.

Noninvasive monitoring of pH in the liquid phase can be accomplished by measuring the colorimetric response of a pH dye using a miniature spectrometer. This method is ratiometric and relatively unaffected by drift. Colorimetric pH sensors also can be implemented as reflective sensors immune to the effects of solution color, sediment or turbidity.

Typically, reflective pH and oxygen sensors comprise patches consisting of an active sensor layer formed by doping a thin sheet of sol gel host matrix with a) target-sensitive fluorophores for oxygen sensing or b) indicator dyes for pH sensing. In some pH sensors a gold mesh can improve reflectivity and be customized for transmission levels, pore sizes, thicknesses and other parameters. As shown in Figure 1, the pH sensor layer is sandwiched between this mesh layer and a layer of adhesive that allows the patch to be affixed to the vessel wall. Oxygen sensor patches can be left bare or coated with a silicone overcoat to make them more robust for harsh environments. [Please note: We now have available updated reflective pH patches.]

Reflective pH patch construction

pH patches

 

Figure 1: Ocean Optics optical pH sensors include an electroformed gold mesh applied atop the sensor layer (inset), which both admits the sample and reflects the probe signal.

Real-time, Noninvasive Monitoring of Oxygen and pH

Bioflask O2 and pH Setup

Figure 2: In this bioflask setup, we used oxygen-sensitive and pH-sensitive patches to monitor changes in the bioreactor during E. coli fermentation.

To monitor oxygen and pH in the liquid phase during E. coli fermentation, oxygen- and pH-sensitive adhesive patches were placed inside a bioflask. The setup used for these measurements is shown in Figure 2.

The fermentation reaction was accomplished using E. coli AG1 Competent Cells from Agilent Technologies, grown overnight at room temperature in LB broth growth medium. E. coli K12 growth medium supplemented with glucose, magnesium and thiamine was used as the medium for the fermentation reaction. To initiate the fermentation reaction, exponentially growing E. coli cells were added to the bioflask containing the K12 growth medium and nutrients.

RedEye® oxygen patches and pH patches were applied to the inside wall of the bioflask. The oxygen patches were attached to the container to monitor oxygen in the liquid phase of the bioflask (patches also could be placed in the headspace if desired). The pH patches were placed near the bottom of the bioflask to minimize the volume of buffer needed for calibration.

Oxygen was measured with a phase fluorometer equipped with a bifurcated optical fiber for the excitation and detection of fluorescence from the RedEye patches. Prior to adding the growth medium to the bioflask, the RedEye patches were calibrated at 0% and 20.9%. The fibers were situated normal to the outside surface of the flask, pointing directly at the patch on the inside of the flask.

The pH was measured using a miniature spectrometer with integrated tungsten halogen light source and a bifurcated optical fiber. One leg of the fiber transmitted light to the patch inside the container and the other leg read the response from the reflective patch inside the solution. Standard pH buffers were used for calibration. Absorbance curves were observed over time.

Results

Oxygen consumption was fastest during the first two hours of the measurements as cell growth increased after the cells were added to fresh culture medium with added nutrients. Oxygen consumption tailed off and began to stabilize through the next six hours of the fermentation process (Figure 3). Since the fermentation reaction was not run in a fully controlled environment, changes observed during the overnight period of the process (hours 8-18) may have been caused by fluctuations in the ambient environment.

Figure 3: Oxygen consumption during E. coli fermentation was fastest during the first two hours of the reaction and stabilized over the next six hours.

Figure 3: Oxygen consumption during E. coli fermentation was fastest during the first two hours of the reaction and stabilized over the next six hours.

As shown in Figure 4, over the 21-hour course of the experiment the pH dropped as cell growth increased, stimulated by exposure to fresh medium and nutrients. The pH dropped because the increased cell growth resulted in a buildup of metabolic by-products like lactic acid.

A more significant drop in pH observed between the 8th and 14th hours of the measurement may have been a function of ambient temperature increase, which increased cell growth as the temperature approached the optimal growth conditions for the cells. The tailing off in pH change observed at the end of the fermentation process was likely due to cell toxicity resulting from the buildup of lactic acid in the culture and depletion of critical culture medium components resulting in slowed cell growth.

An optical pH sensor monitors pH during E. coli fermentation in a bioreactor environment.

Figure 4: As the E. coli fermentation experiment progressed, pH levels began to drop, most likely as a result of increases in ambient temperatures and lactic acid.

Conclusions

The limitations of electrochemical-based oxygen and pH sensing are overcome by optical oxygen and pH patches from Ocean Optics. Such patches can be integrated easily within a small-scale biosystem such as a bioflask and provide continuous, non-intrusive monitoring of key system parameters. The ability to monitor DO and pH in real time without perturbing a sealed environment can lead to an improved understanding of the processes in the bioreactor and, ultimately, help to facilitate the development of new biological products and processes.

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